BONE REGENERATION IN A SYNTETIC 3D MODEL

Background: Adipose mesenchymal stem cell (AMSC) condensation contributes to differentiation in bone tissue. This condensation is characterized by the development of high concentration cell masses, which appear during skeletogenesis. The progress of tissue engineering, in combination with the rising of regenerative medicine, yields new methods as the use of new elements to engage bone generation arises. Methods: We developed a new approach to generate bone tissue. Osseous tissue regeneration was assessed using AMSC-spheroid, extracellular matrix (EM), BMPs, TGF-B, FGF, IGF and PDGF, applied on synthetic 3D printed vertebrae (n=17). We established two study groups; the first was treated with AMSC-spheroid, EM, and growth factors; the second was the control group. Depending on the conditions, we used one-way repeated-measure ANOVAs. Results: Two weeks after AMSC, EM and growth factor applications, bone generation was detected (n=17) in 35% of vertebral surfaces; four weeks after treatment, vertebrae were concealed with bone tissue in 65% of their surfaces. In the second group (n=17), 100% of synthetic models displayed no bone formation (p=0,001). Conclusion: This new approach could be the initial step towards the potential solution for several osseous illnesses as a bone graft substitute.