The Effects of Chelation Therapy on Catalase Activity and the Implications in Neurodegener

Friday, February 12, 2016
Drew Brown, Grove High School, Grove, OK
The purpose of this project is to determine if chelation therapy effects catalase activity in a Invivo experiment and to observe vitamin C’s ability to stabilize the catalase reaction during chelation therapy.  Phase II found that chelation therapy slows down catalase activity and vitamin C brings the catalase activity back to normal levels. Once catalase activity is slowed down hydrogen peroxide accumulates and generates free radicals which are known to be a precursor to neurodegeneration. The hypothesis stated: EDTA chelation therapy will inhibit the catalase reaction in C. elegans. It was further hypothesized that vitamin c supplementation will stabilize the catalase reaction during EDTA chelation therapy. To test the hypotheses a series of enzyme and protein assays were utilized. 12 plates of C. elegans were cultures and 3 were left as a control, 3 were exposed to lead, 3 were exposed to lead and EDTA, and the final 3 were exposed to lead/EDTA/vitamin C. Worms were allowed to grow then washed off plates and homogenized using a bead blender, then a protein assay was ran on all samples and protein content factored into catalase activity equation. Finally a catalase enzyme assay was ran on all samples to observe reaction rate in nmol/min/ml. During this experiment it was found that chelation therapy slows catalase activity by 7.2nmol/min/ml, and the addition of vitamin C further slowed the reaction down by an additional 16.44nmol/min/ml.