DECELLULARIZATION AND REHABILITATION OF THE URINARY BLADDER

Background: Urinary bladder regeneration based on tissue engineering approaches is crucial in reconstructive therapy. The urinary bladder is a multifaceted organ, and there are several clinical illnesses that demand its substitution. Refinement of biotechnology, in combination with the rising of regenerative medicine, yields engaging new approaches to secure bladder generation. Methods: We treated 30 urinary bladder using Triton X-100 with ammonium dioxide to complete bladder decellularization. The healing and regenerative potentials of adipose derived stem cells (ADSC) and growth factors were assessed using these elements in decellularizated bladders (n=30). We established three study groups; the first treated with ADSC + VEGF (n=15), second with ADSC + VEGF + bFGF (n=15), and the third was the control group (n=15). Depending on the conditions, we would utilize one-way repeated-measures ANOVAs. Results: 8 weeks after the ADSC + VEGF + bFGF application, we observed a complete regenerated urothelium and smooth muscle layer in all the participants (n=15) in the second group. While in the first group (n=15), 80% of animals had a poor organization of smooth muscle layers at 8 weeks (p=0,001). Conclusion: Bladder acellular matrix has potential application for urinary bladder regeneration associated with ADSC plus VEGF + bFGF, because it decreases the time of bladder regeneration.